Flash chromatography is a chemical separation method used to purify or isolate different liquid substances. The mixture is driven through a column with low pressure. This causes different degrees of absorption of the constituents at the separation column due to which the mixture is disaggregated.
A UV-VIS lamp can be used to identify the constituents. The lamp is integrated in a UV-VIS detector. All substances have specific absorption patterns. The detector records the absorption patterns of the different substances after they have passed the separation column, which enables unambiguous identification and separation of the different constituents.
This analytic separation method is used in particular in industrial applications and in biology, e.g. to separate amino acids.