High Pressure Liquid Chromatography (HPLC+UHPLC)

High performance liquid chromatography

High-pressure liquid chromatography is an analytical technique used to check the purity of pharmaceutical drugs and detect contamination in food. Noblelight deuterium lamps provide the light sources for these highly sensitive analyses and help determine traces of undesired contamination such as melamine in milk powder for babies.

HPLC is based on a separation method in which the substance to be examined is pumped through a so-called separating column at high pressure. Due to the different kinds of interaction of the substances with the materials in the separating column, the individual substances exit the column at different times, which enables separation of the fractions. The substance, which is now broken up into its individual constituents, can be detected by a UV-Vis detector that measures absorption in the UV range.